a rapid and simple method of dna extraction from clinical specimens containing mycobacterium leprae for pcr assays

نویسندگان

abdolnasser rapi from the department of medical microbiology, ucl medical school, university of london, u.k

mohammed h. mobayen the baba baghi leprosy hospital, tabriz, islamic republic of iran.

francois feval the baba baghi leprosy hospital, tabriz, islamic republic of iran.

john l. stanford from the department of medical microbiology, ucl medical school, university of london, u.k

چکیده

we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase chain reaction (pcr) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kda antigen of m. leprae. all the specimens were found to be pcr -positive, suggesting the efficiency of the present dna extraction method.

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عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۰، شماره ۳، صفحات ۲۳۳-۲۳۵

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